← Articles

Peng et al: Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Veterinary Surgery 3, 2025

🔍 Key Findings

Objective: Determine if topical 45 mg/mL amikacin in CMC gel leads to systemic absorption in dogs with wounds.

Dogs enrolled: 11 client-owned dogs, with 31 applications of the gel.

Serum findings:

  • Only 5 of 153 samples were above the 2.5 µg/mL quantification limit
  • All values remained <5 µg/mL, the presumed toxicity threshold
  • No correlation was found between dose-related parameters (mg, mg/kg, mg/cm²) and serum amikacin levels

Peak concentrations were observed at ~2 hours post-application, declining rapidly thereafter

No nephrotoxicity observed, and most values were below detection

Conclusion: Topical amikacin gel appears safe at doses up to 24.9 mg/kg, with minimal systemic absorption

Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel

Simini Surgery Review Podcast

How critical is this paper for crushing the Boards?

🚨 Must-know. I’d bet on seeing this.

📚 Useful background, not must-know.

💤 Skip it. Doubt it’ll ever show up.

Thanks for the feedback!
‍We'll keep fine-tuning the articles vault.
Oops — didn’t go through.
‍Mind trying that again?
← Articles

Peng et al: Serum amikacin concentrations in dogs with naturally occurring open wounds treated with topical amikacin in carboxymethylcellulose hydrogel
Veterinary Surgery 3, 2025

🔍 Key Findings

Objective: Determine if topical 45 mg/mL amikacin in CMC gel leads to systemic absorption in dogs with wounds.

Dogs enrolled: 11 client-owned dogs, with 31 applications of the gel.

Serum findings:

  • Only 5 of 153 samples were above the 2.5 µg/mL quantification limit
  • All values remained <5 µg/mL, the presumed toxicity threshold
  • No correlation was found between dose-related parameters (mg, mg/kg, mg/cm²) and serum amikacin levels

Peak concentrations were observed at ~2 hours post-application, declining rapidly thereafter

No nephrotoxicity observed, and most values were below detection

Conclusion: Topical amikacin gel appears safe at doses up to 24.9 mg/kg, with minimal systemic absorption

Simini Surgery Review Podcast

Join Now to Access Key Summaries to more Veterinary Surgery Articles!
Log InSign Up

Multiple Choice Questions on this study

In Peng 2025 et al., on topical amikacin gel, what correlation was found between dose and serum levels?

A. Strong positive correlation
B. Weak positive correlation
C. Moderate correlation in large wounds
D. No significant correlation found
E. Negative correlation with dose

Answer: No significant correlation found

Explanation: Serum levels showed no correlation to mg, mg/kg, mg/cm², or other dose parameters【90†Veterinary Surgery†L1-L20】
In Peng 2025 et al., on topical amikacin gel, what maximum topical dose per kg was administered?

A. 10.2 mg/kg
B. 15.8 mg/kg
C. 18.6 mg/kg
D. 24.9 mg/kg
E. 30.0 mg/kg

Answer: 24.9 mg/kg

Explanation: The highest dose applied to any dog was 24.9 mg/kg, nearing the systemic recommended maximum【90†Veterinary Surgery†L1-L20】
In Peng 2025 et al., on topical amikacin gel, what was the highest observed serum amikacin concentration?

A. 2.97 μg/mL
B. 3.82 μg/mL
C. 5.1 μg/mL
D. 6.4 μg/mL
E. 7.0 μg/mL

Answer: 3.82 μg/mL

Explanation: The highest measured serum concentration was 3.82 μg/mL, remaining below the 5 μg/mL toxicity threshold【90†Veterinary Surgery†L1-L20】
In Peng 2025 et al., on topical amikacin gel, what was the lower limit of quantification (LLOQ) for the assay used?

A. 0.5 μg/mL
B. 1.0 μg/mL
C. 2.0 μg/mL
D. 2.5 μg/mL
E. 5.0 μg/mL

Answer: 2.5 μg/mL

Explanation: The Siemens Syva EMIT Amikacin Assay used in this study had an LLOQ of 2.5 μg/mL【90†Veterinary Surgery†L1-L20】
In Peng 2025 et al., on topical amikacin gel, at what time point were serum levels consistently above the lower limit of quantification (LLOQ)?

A. Immediately after application
B. 1 hour post-application
C. 2 hours post-application
D. 6 hours post-application
E. 24 hours post-application

Answer: 2 hours post-application

Explanation: All detectable serum concentrations >2.5 μg/mL occurred at ~2 hours post-application【90†Veterinary Surgery†L1-L20】

Elevate Your Infection Control Protocol

Implement Simini Protect Lavage for superior, clinically-proven post-operative skin antisepsis and reduced infection risk.

Start Your Free Trial TodayStart Your Free Trial

Review More Key Summaries from this Veterinary Surgery Journal Issue

Price

 et al: 

Evaluation of thoracic duct ligation and unilateral subphrenic pericardiectomy via a left fourth intercostal approach in normal canine cadavers

Smith

 et al: 

Comparison of bacteria cultured during a total ear canal ablation and subsequent incisional dehiscence in 12 dogs

Heald

 et al: 

Electroceutical treatment of infected chronic wounds in a dog and a cat

Caiazzo

 et al: 

Impact of suture materials polydioxanone and poliglecaprone 25 on non-infected clean orthopedic stifle procedure incisional dehiscence rates

Drudi

 et al: 

Comparison of immediate and short‐term outcomes of cricoarytenoid and thyroarytenoid lateralization in dogs with idiopathic laryngeal paralysis